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Custom made rabbit anti-zebrafish LMLN antibodies

Genosphere Biotechnologies

Bioz Stars: 4.09

First Appeared: 2013
Perturbation of invadolysin disrupts cell migration... (Exp Cell Res 2013 May 01)

"... antibodies (A6970 and A6971) raised against peptide sequence YCDSVRSAPLQLTC (Genosphere Biotechnologies) which were used at 1:250 dilution ..."

"The fish were then washed 3×5 min in PBS-Tx and the secondary antibodies (plus 0.5 μg/ml of DAPI if using) were added (diluted in PBS-Tx) and incubated at room temperature for 2 h.

The fish were then washed 4×5 min in PBS-Tx, and finally mounted in PBS containing 50% glycerol.. The primary antibodies used in this work include: two custom made rabbit anti-zebrafish LMLN antibodies (A6970 and A6971) raised against peptide sequence YCDSVRSAPLQLTC (Genosphere Biotechnologies) which were used at 1:250 dilution, and mouse anti-acetylated tubulin clone 6–11 B-1 (Sigma) used at 1:500 dilution.

Secondary antibodies were diluted at 1:500 and include: Alexa Fluor 594 donkey anti-rabbit IgG (A21…