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Custom made rabbit anti-zebrafish LMLN antibodies

Genosphere Biotechnologies

Bioz Stars: 4.09

First Appeared: 2013

Perturbation of invadolysin disrupts cell migration... (Exp Cell Res 2013 May 01)


"... antibodies (A6970 and A6971) raised against peptide sequence YCDSVRSAPLQLTC (Genosphere Biotechnologies) which were used at 1:250 dilution ..."


"The fish were then washed 3×5 min in PBS-Tx and the secondary antibodies (plus 0.5 μg/ml of DAPI if using) were added (diluted in PBS-Tx) and incubated at room temperature for 2 h.

The fish were then washed 4×5 min in PBS-Tx, and finally mounted in PBS containing 50% glycerol.. The primary antibodies used in this work include: two custom made rabbit anti-zebrafish LMLN antibodies (A6970 and A6971) raised against peptide sequence YCDSVRSAPLQLTC (Genosphere Biotechnologies) which were used at 1:250 dilution, and mouse anti-acetylated tubulin clone 6–11 B-1 (Sigma) used at 1:500 dilution.

Secondary antibodies were diluted at 1:500 and include: Alexa Fluor 594 donkey anti-rabbit IgG (A21206), Alexa Fluor 488 donkey anti-mouse IgG (A21202), and Alexa Fluor 488 Phalloidin (A12379)—all from Invitrogen."


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GHK-Cu Peptide : Anwendungen in der Wundtherapie und der Kosmetik


Wound healing activity of GHK-Cu was confirmed in animal experiments. GHK-Cu accelerated wound healing and increased blood vessel formation and the level of antioxidant enzymes in rabbits. This molecule also induced systemic wound healing in rats, mice, and pigs. It improved the healing of diabetic and ischemic wounds in rats, decreasing the level of TNF-alpha and stimulating collagen synthesis. It also facilitated healing of pad wounds in dogs [1217]. Such well-documented skin regeneration activity prompted widespread use of GHK in antiaging cosmetic products [18].


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http://www.biomodul.de/sitedirectedmutagenesis.html


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http://www.biomodul.de/CustomPeptideSynthesis.pdf