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endotoxinfreie DNA

Gensynthese Service

Ablaufplanung eines Gen-Synthese Projekts:

1. Codon Optimierung (falls notwendig).
2. Sequenzdesign: Hinzufügen oder entfernen von besonderen DNA-Sequenzmerkmalen einschließlich N- oder C-terminalen Reinigungstags (z.B. His6tag, GST), entfernen von Restriktions-Schnittstellen, etc. 3. Vollständige de novo Gensynthese, genau Ihren Anforderungen entsprechend. 4. Klonierung, Screening, Vervielfältigung und Reinigung ihres Klons. Die Klonierung der DNA erfolgt üblicherweise in unser pUC-Plasmid, Sie können uns jedoch hierfür auch ein anderes Plasmid (pET usw.) für die Subklonierung zur Verfügung stellen.

5. Die Richtigkeit der Gensequenz wird durch die vollständige doppelsträngige DNA-Sequenzierung nachgewiesen.
Sie erhalten standardmäßig 5 µg der gereinigten klonierten pUC Plasmid-DNA. Gerne stellen wir für Sie auch größere Mengen (endotoxinfreier) Plasmid-DNA her. 
LF






Custom made rabbit anti-zebrafish LMLN antibodies

Genosphere Biotechnologies

Bioz Stars: 4.09

First Appeared: 2013
Perturbation of invadolysin disrupts cell migration... (Exp Cell Res 2013 May 01)

"... antibodies (A6970 and A6971) raised against peptide sequence YCDSVRSAPLQLTC (Genosphere Biotechnologies) which were used at 1:250 dilution ..."

"The fish were then washed 3×5 min in PBS-Tx and the secondary antibodies (plus 0.5 μg/ml of DAPI if using) were added (diluted in PBS-Tx) and incubated at room temperature for 2 h.

The fish were then washed 4×5 min in PBS-Tx, and finally mounted in PBS containing 50% glycerol.. The primary antibodies used in this work include: two custom made rabbit anti-zebrafish LMLN antibodies (A6970 and A6971) raised against peptide sequence YCDSVRSAPLQLTC (Genosphere Biotechnologies) which were used at 1:250 dilution, and mouse anti-acetylated tubulin clone 6–11 B-1 (Sigma) used at 1:500 dilution.

Secondary antibodies were diluted at 1:500 and include: Alexa Fluor 594 donkey anti-rabbit IgG (A21…

mouse, rat, guinea pigs - antibodies production

We offer a range of high-quality custom antibody production services.
To learn more about our different antibody services, please select your service of interest below. 

http://www.biomodul.de/maus.html

http://www.biomodul.de/ratte.html

http://www.biomodul.de/guinea_pig.html

Referenzen:

https://www.bioz.com/search?utf8=%E2%9C%93&q=Genosphere&other=true&exact_search=true&rating_sort=true#page-1
LF

Peptide and Gene Synthesis Service

Custom Service for Research

GHK-Cu Peptide : Anwendungen in der Wundtherapie und der Kosmetik


Wound healing activity of GHK-Cu was confirmed in animal experiments. GHK-Cu accelerated wound healing and increased blood vessel formation and the level of antioxidant enzymes in rabbits. This molecule also induced systemic wound healing in rats, mice, and pigs. It improved the healing of diabetic and ischemic wounds in rats, decreasing the level of TNF-alpha and stimulating collagen synthesis. It also facilitated healing of pad wounds in dogs [1217]. Such well-documented skin regeneration activity prompted widespread use of GHK in antiaging cosmetic products [18].


LF


Gensynthese Service http://www.biomodul.de/Gene.html

Site directed mutagenesis Service: Insertion, Deletion und Substitution.
http://www.biomodul.de/sitedirectedmutagenesis.html


Peptidsynthese Service
http://www.biomodul.de/CustomPeptideSynthesis.pdf





Genosphere Biotechnologies 38

Genosphere Biotechnologies vous propose des services et des produits de la plus haute qualité, pour vos projet de recherche. Avec une équipe expérimentée de chimistes organiciens, de biochimistes et d'immuniochimistes, nous vous assurons d'un coût intéressant, un contrôle qualité rigoureux et extensif et un service clients cordial et efficace.


LF